Interaction information

Interaction summary

Short label: TRANSLATION INITIATION FACTOR-16S RIBOSOMAL RNA
Full name: TRANSLATION INITIATION FACTOR interacts with 16S RIBOSOMAL RNA
Source database: RCSB PDB
ID: 1HR0
Date modified: 2011-03-24 01:41:29 JST

Protein information

Short label

TRANSLATION INITIATION FACTOR

Full name

Chain W of model 1HR0: TRANSLATION INITIATION FACTOR

Origin

Escherichia coli

NCBI Taxonomy ID

562

Name

Escherichia coli

Synonyms

16 synonym(s).

"Bacillus coli" Migula 1895
"Bacterium coli commune" Escherich 1885
"Bacterium coli" (Migula 1895) Lehmann and Neumann 1896
Bacillus coli
Bacterium coli
Bacterium coli commune
Enterococcus coli
Escherchia coli
Escherichia coli (Migula 1895) Castellani and Chalmers 1919
Escherichia coli retron Ec107
Escherichia coli retron Ec67
Escherichia coli retron Ec79
Escherichia coli retron Ec86
Eschericia coli
bacterium 10a
bacterium E3

Sequence

1HR0W:TRANSLATION INITIATION FACTOR

RNA information

Short label

16S RIBOSOMAL RNA

Full name

Chain A of model 1HR0: 16S RIBOSOMAL RNA

Cross references

1 item(s)

GenBank Nucleotide: 155076

Sequence

1HR0A:16S RIBOSOMAL RNA

Experiments (1)

Experiment 1

carter-2001

Full name

X-ray diffraction

Detection method

x-ray crystallography (MI:0114)

Term ID

MI:0114

Name

x-ray crystallography

Definition

Analysis of a diffraction pattern generated by a single crystal. X-rays have a wavelength, typically around 1 Angstrom (the diameter of a hydrogen atom). If a narrow parallel beam of X-rays is directed at a sample of a pure protein, most of the X-rays will pass straight through it. A small fraction, however, will be scattered by the atoms in the sample. If the sample is a well-ordered crystal, the scattered waves will reinforce one another at certain points and will appear as diffraction spots when the X-rays are recorded by a suitable detector. The position and intensity of each spot in the X-ray diffraction pattern contain information about the position and nature of the atoms in the crystal. The three-dimensional structure of a large molecule can be deduced from the electron-density map of its crystal. In recent years X-ray diffraction analysis has become increasingly automated, and now the slowest step is likely to be the production of suitable macromolecule crystals. This requires high concentration of very pure macromolecule and empirical searching for the proper crystallisation conditions.

Synonyms

2 synonym(s)

X-ray (PSI-MI-alternate)
x-ray diffraction (PSI-MI-short)

Is-a

2 item(s)

MI:0013: biophysical
MI:0659: experimental feature detection

Host organisms

1 host organism(s)

Origin

Escherichia coli

NCBI Taxonomy ID

562

Name

Escherichia coli

Synonyms

16 synonym(s).

"Bacillus coli" Migula 1895
"Bacterium coli commune" Escherich 1885
"Bacterium coli" (Migula 1895) Lehmann and Neumann 1896
Bacillus coli
Bacterium coli
Bacterium coli commune
Enterococcus coli
Escherchia coli
Escherichia coli (Migula 1895) Castellani and Chalmers 1919
Escherichia coli retron Ec107
Escherichia coli retron Ec67
Escherichia coli retron Ec79
Escherichia coli retron Ec86
Eschericia coli
bacterium 10a
bacterium E3

Reference

CARTER, A P, et al., Science 2001 Jan;291(5503):498-501

PMID

11228145

Title

Crystal structure of an initiation factor bound to the 30S ribosomal subunit.

Authors

CARTER, A P, et al.

CARTER, A P
CLEMONS, W M
BRODERSEN, D E
MORGAN-WARREN, R J
HARTSCH, T
WIMBERLY, B T
RAMAKRISHNAN, V

Citation

Science 2001 Jan;291(5503):498-501

References (1)

Reference 1

CARTER, A P, et al., Science 2001 Jan;291(5503):498-501

PMID

11228145

Title

Crystal structure of an initiation factor bound to the 30S ribosomal subunit.

Authors

CARTER, A P, et al.

CARTER, A P
CLEMONS, W M
BRODERSEN, D E
MORGAN-WARREN, R J
HARTSCH, T
WIMBERLY, B T
RAMAKRISHNAN, V

Citation

Science 2001 Jan;291(5503):498-501

Attributes (3)

Attributes

3 attribute(s)

3d-structure: CRYSTAL STRUCTURE OF INITIATION FACTOR IF1 BOUND TO THE 30S RIBOSOMAL SUBUNIT
3d-resolution: 3.20A
3d-r-factors: working 21.780%, free 26.090%

Interaction information