Your browser does not support JavaScript or JavaScript is disabled. This site does not function correctly with JavaScript disabled.
Interaction information
Interaction summary
Short label
cte-dhx9-11
Full name
RNA-selection : Biotinylated SRV-1 viral CTE RNA trapped RNA helicase A
Source database
IntAct ID
Type
colocalization (MI:0403)
Term ID
Name
colocalization
Definition
Coincident occurrence of molecules in a given subcellular fraction observed with a low resolution methodology from which a physical interaction among those molecules cannot be inferred.
Is-a
1 item(s)
MI:0190
interaction type
Date modified
2011-03-19 22:53:20 JST
Protein information
Short label
dhx9_human
Full name
ATP-dependent RNA helicase A
Gene
DHX9 (DEAH (Asp-Glu-Ala-His) box polypeptide 9)
GeneID
Official symbol
DHX9
Synonyms
5 synonym(s)
Description
DEAH (Asp-Glu-Ala-His) box polypeptide 9
Other names
5 designation(s)
ATP-dependent RNA helicase A
DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9
DEAH box protein 9
leukophysin
nuclear DNA helicase II
Ontologies
19 ontology(s)
Functions
9 function(s)
GO:0000166
[IEA] nucleotide binding
Evidence
IEA Term ID
Name
nucleotide binding
Namespace
molecular_function
Definition
Interacting selectively and non-covalently with a nucleotide, any compound consisting of a nucleoside that is esterified with (ortho)phosphate or an oligophosphate at any hydroxyl group on the ribose or deoxyribose moiety.
Is-a
1 item(s)
GO:0005488
binding
GO:0003677
[IEA] DNA binding
Evidence
IEA Term ID
Name
DNA binding
Namespace
molecular_function
Definition
Any molecular function by which a gene product interacts selectively with DNA (deoxyribonucleic acid).
Synonyms
2 synonym(s)
microtubule/chromatin interaction
plasmid binding
Is-a
1 item(s)
GO:0003676
nucleic acid binding
GO:0003724
[TAS] RNA helicase activity
Evidence
TAS References
1 reference(s)
PubMed
ABDELHALEEM, M M, et al. , J. Immunol. 1996 Mar;156(6):2026-35
PMID
Title
Leukophysin: an RNA helicase A-related molecule identified in cytotoxic T cell granules and vesicles.
Authors
ABDELHALEEM, M M, et al.
ABDELHALEEM, M M
HAMEED, S
KLASSEN, D
GREENBERG, A H
Citation
J. Immunol. 1996 Mar;156(6):2026-35
Term ID
Name
RNA helicase activity
Namespace
molecular_function
Definition
Catalysis of the reaction: NTP + H2O = NDP + phosphate to drive the unwinding of a RNA helix.
Is-a
1 item(s)
GO:0004386
helicase activity
GO:0003725
[IEA] double-stranded RNA binding
Evidence
IEA Term ID
Name
double-stranded RNA binding
Namespace
molecular_function
Definition
Interacting selectively and non-covalently with double-stranded RNA.
Synonyms
1 synonym(s)
Is-a
1 item(s)
GO:0003723
RNA binding
GO:0004003
[TAS] ATP-dependent DNA helicase activity
Evidence
TAS References
1 reference(s)
PubMed
ZHANG, S, et al. , J. Biol. Chem. 1997 Apr;272(17):11487-94
PMID
Title
Domain structure of human nuclear DNA helicase II (RNA helicase A).
Authors
ZHANG, S, et al.
Citation
J. Biol. Chem. 1997 Apr;272(17):11487-94
Term ID
Name
ATP-dependent DNA helicase activity
Namespace
molecular_function
Definition
Catalysis of the reaction: ATP + H2O = ADP + phosphate, driving the unwinding of the DNA helix.
Is-a
3 item(s)
GO:0003678
DNA helicase activity
GO:0008026
ATP-dependent helicase activity
GO:0008094
DNA-dependent ATPase activity
GO:0004004
[TAS] ATP-dependent RNA helicase activity
Evidence
TAS References
1 reference(s)
PubMed
ZHANG, S, et al. , J. Biol. Chem. 1997 Apr;272(17):11487-94
PMID
Title
Domain structure of human nuclear DNA helicase II (RNA helicase A).
Authors
ZHANG, S, et al.
Citation
J. Biol. Chem. 1997 Apr;272(17):11487-94
Term ID
Name
ATP-dependent RNA helicase activity
Namespace
molecular_function
Definition
Catalysis of the reaction: ATP + H2O = ADP + phosphate, driving the unwinding of an RNA helix.
Is-a
3 item(s)
GO:0003724
RNA helicase activity
GO:0008026
ATP-dependent helicase activity
GO:0008186
RNA-dependent ATPase activity
GO:0005515
[IPI] protein binding
Evidence
IPI References
1 reference(s)
PubMed
TANG, H, et al. , J. Biol. Chem. 2000 Oct;275(42):32694-700
PMID
Title
Specific interaction between RNA helicase A and Tap, two cellular proteins that bind to the constitutive transport element of type D retrovirus.
Authors
TANG, H, et al.
Citation
J. Biol. Chem. 2000 Oct;275(42):32694-700
Term ID
Name
protein binding
Namespace
molecular_function
Definition
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
Synonyms
5 synonym(s)
alpha-2 macroglobulin receptor-associated protein activity
protein amino acid binding
protein degradation tagging activity
protein folding chaperone (GOC:dph, GOC:mah, GOC:tb)
protein tagging activity
Is-a
1 item(s)
GO:0005488
binding
GO:0005524
[IEA] ATP binding
Evidence
IEA Term ID
Name
ATP binding
Namespace
molecular_function
Definition
Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator." [ISBN:0198506732 "Oxford Dictionary of Biochemistry and Molecular Biology
Is-a
1 item(s)
GO:0032559
adenyl ribonucleotide binding
GO:0016787
[IEA] hydrolase activity
Evidence
IEA Term ID
Name
hydrolase activity
Namespace
molecular_function
Definition
Catalysis of the hydrolysis of various bonds, e.g. C-O, C-N, C-C, phosphoric anhydride bonds, etc. Hydrolase is the systematic name for any enzyme of EC class 3." [ISBN:0198506732 "Oxford Dictionary of Biochemistry and Molecular Biology
Is-a
1 item(s)
GO:0003824
catalytic activity
Processes
3 process(s)
GO:0008380
[EXP] RNA splicing
Evidence
EXP References
1 reference(s)
PubMed
ZHOU, Zhaolan, et al. , Nature 2002 Sep;419(6903):182-5
PMID
Title
Comprehensive proteomic analysis of the human spliceosome.
Authors
ZHOU, Zhaolan, et al.
ZHOU, Zhaolan
LICKLIDER, Lawrence J
GYGI, Steven P
REED, Robin
Citation
Nature 2002 Sep;419(6903):182-5
Term ID
Name
RNA splicing
Namespace
biological_process
Definition
The process of removing sections of the primary RNA transcript to remove sequences not present in the mature form of the RNA and joining the remaining sections to form the mature form of the RNA.
Synonyms
1 synonym(s)
pre-mRNA splicing factor activity
Is-a
1 item(s)
GO:0006396
RNA processing
GO:0034605
[IEA] cellular response to heat
Evidence
IEA Term ID
Name
cellular response to heat
Namespace
biological_process
Definition
A change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a heat stimulus, a temperature stimulus above the optimal temperature for that organism.
Synonyms
1 synonym(s)
cellular response to heat stress
Is-a
2 item(s)
GO:0009408
response to heat
GO:0033554
cellular response to stress
GO:0070934
[IMP] CRD-mediated mRNA stabilization
Evidence
IMP References
1 reference(s)
PubMed
WEIDENSDORFER, Doreen, et al. , RNA 2009 Jan;15(1):104-15
PMID
Title
Control of c-myc mRNA stability by IGF2BP1-associated cytoplasmic RNPs.
Authors
WEIDENSDORFER, Doreen, et al.
WEIDENSDORFER, Doreen
STöHR, Nadine
BAUDE, Anne
LEDERER, Marcell
KöHN, Marcel
SCHIERHORN, Angelika
BUCHMEIER, Sabine
WAHLE, Elmar
HüTTELMAIER, Stefan
Citation
RNA 2009 Jan;15(1):104-15
Term ID
Name
CRD-mediated mRNA stabilization
Namespace
biological_process
Definition
An mRNA stabilization process in which one or more RNA-binding proteins associate with a sequence in the open reading frame called the coding region instability determinant (CRD).
Synonyms
1 synonym(s)
coding region determinant-mediated mRNA stabilization (GOC:mah)
Is-a
1 item(s)
GO:0048255
mRNA stabilization
Components
7 component(s)
GO:0005622
[IEA] intracellular
Evidence
IEA Term ID
Name
intracellular
Namespace
cellular_component
Definition
The living contents of a cell; the matter contained within (but not including) the plasma membrane, usually taken to exclude large vacuoles and masses of secretory or ingested material. In eukaryotes it includes the nucleus and cytoplasm." [ISBN:0198506732 "Oxford Dictionary of Biochemistry and Molecular Biology
Synonyms
4 synonym(s)
internal to cell
nucleocytoplasm (GOC:mah)
protoplasm
protoplast (GOC:mah)
Is-a
1 item(s)
GO:0044464
cell part
GO:0005634
[IDA] nucleus
Evidence
IDA References
1 reference(s)
PubMed
ZHANG, Suisheng, et al. , Cell Biol. Int. 2007 Oct;31(10):1109-21
PMID
Title
Werner syndrome helicase (WRN), nuclear DNA helicase II (NDH II) and histone gammaH2AX are localized to the centrosome.
Authors
ZHANG, Suisheng, et al.
ZHANG, Suisheng
HEMMERICH, Peter
GROSSE, Frank
Citation
Cell Biol. Int. 2007 Oct;31(10):1109-21
Term ID
Name
nucleus
Namespace
cellular_component
Definition
A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
Synonyms
1 synonym(s)
Is-a
1 item(s)
GO:0043231
intracellular membrane-bounded organelle
GO:0005730
[IEA] nucleolus
Evidence
IEA Term ID
Name
nucleolus
Namespace
cellular_component
Definition
A small, dense body one or more of which are present in the nucleus of eukaryotic cells. It is rich in RNA and protein, is not bounded by a limiting membrane, and is not seen during mitosis. Its prime function is the transcription of the nucleolar DNA into 45S ribosomal-precursor RNA, the processing of this RNA into 5.8S, 18S, and 28S components of ribosomal RNA, and the association of these components with 5S RNA and proteins synthesized outside the nucleolus. This association results in the formation of ribonucleoprotein precursors; these pass into the cytoplasm and mature into the 40S and 60S subunits of the ribosome." [ISBN:0198506732 "Oxford Dictionary of Biochemistry and Molecular Biology
Is-a
2 item(s)
GO:0043232
intracellular non-membrane-bounded organelle
GO:0044428
nuclear part
GO:0005737
[IEA] cytoplasm
Evidence
IEA Term ID
Name
cytoplasm
Namespace
cellular_component
Definition
All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
Is-a
1 item(s)
GO:0044424
intracellular part
GO:0005813
[IDA] centrosome
Evidence
IDA References
1 reference(s)
PubMed
ZHANG, Suisheng, et al. , Cell Biol. Int. 2007 Oct;31(10):1109-21
PMID
Title
Werner syndrome helicase (WRN), nuclear DNA helicase II (NDH II) and histone gammaH2AX are localized to the centrosome.
Authors
ZHANG, Suisheng, et al.
ZHANG, Suisheng
HEMMERICH, Peter
GROSSE, Frank
Citation
Cell Biol. Int. 2007 Oct;31(10):1109-21
Term ID
Name
centrosome
Namespace
cellular_component
Definition
A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle.
Is-a
2 item(s)
GO:0005815
microtubule organizing center
GO:0043232
intracellular non-membrane-bounded organelle
GO:0030529
[IDA] ribonucleoprotein complex
Evidence
IDA References
1 reference(s)
PubMed
JøNSON, Lars, et al. , Mol. Cell Proteomics 2007 May;6(5):798-811
PMID
Title
Molecular composition of IMP1 ribonucleoprotein granules.
Authors
JøNSON, Lars, et al.
JøNSON, Lars
VIKESAA, Jonas
KROGH, Anders
NIELSEN, Lars K
HANSEN, Thomas vO
BORUP, Rehannah
JOHNSEN, Anders H
CHRISTIANSEN, Jan
NIELSEN, Finn C
Citation
Mol. Cell Proteomics 2007 May;6(5):798-811
Term ID
Name
ribonucleoprotein complex
Namespace
cellular_component
Definition
A macromolecular complex containing both protein and RNA molecules.
Synonyms
4 synonym(s)
protein-RNA complex
ribonucleoprotein
RNA-protein complex
RNP
Is-a
2 item(s)
GO:0032991
macromolecular complex
GO:0044424
intracellular part
GO:0070937
[IDA] CRD-mediated mRNA stability complex
Evidence
IDA References
1 reference(s)
PubMed
WEIDENSDORFER, Doreen, et al. , RNA 2009 Jan;15(1):104-15
PMID
Title
Control of c-myc mRNA stability by IGF2BP1-associated cytoplasmic RNPs.
Authors
WEIDENSDORFER, Doreen, et al.
WEIDENSDORFER, Doreen
STöHR, Nadine
BAUDE, Anne
LEDERER, Marcell
KöHN, Marcel
SCHIERHORN, Angelika
BUCHMEIER, Sabine
WAHLE, Elmar
HüTTELMAIER, Stefan
Citation
RNA 2009 Jan;15(1):104-15
Term ID
Name
CRD-mediated mRNA stability complex
Namespace
cellular_component
Definition
A protein complex that binds to, and promotes stabilization of, mRNA molecules containing the coding region instability determinant (CRD). In human, IGF2BP1 and at least four additional proteins: HNRNPU, SYNCRIP, YBX1, and DHX9.
Is-a
2 item(s)
GO:0043234
protein complex
GO:0044444
cytoplasmic part
Origin
Homo sapiens
NCBI Taxonomy ID
Name
Homo sapiens
Synonyms
2 synonym(s).
Cross references
34 item(s)
UniProt
Gene Ontology
Gene Ontology
Gene Ontology
IntAct
Gene Ontology
RefSeq
Reactome
Reactome
Gene Ontology
Gene Ontology
InterPro
InterPro
InterPro
InterPro
InterPro
UniProt
Gene Ontology
InterPro
InterPro
UniProt
UniProt
Gene Ontology
Reactome
Ensembl
InterPro
InterPro
International Protein Index
Gene Ontology
Gene Ontology
Gene Ontology
UniProt
Gene Ontology
IntAct
Experimental roles
1 role(s)
Role 1
neutral component
Term ID
Name
neutral component
Definition
Molecule role in an experimental setting that does not have an embedded asymmetry.
Is-a
1 item(s)
MI:0495
experimental role
Sequence
RNA information
Short label
cte_srv1
Full name
constitutive transport element (CTE) in the 3'UTR of Simian SRV-1 type D retrovirus
Origin
Simian retrovirus 1
NCBI Taxonomy ID
Name
Simian retrovirus 1
Synonyms
5 synonym(s).
SRV-1
Simian AIDS retrovirus SRV-1
Simian SRV-1 type D retrovirus
Simian retrovirus SRV-1
Simian type D virus 1
Cross references
2 item(s)
INSDC
IntAct
Experimental roles
1 role(s)
Role 1
neutral component
Term ID
Name
neutral component
Definition
Molecule role in an experimental setting that does not have an embedded asymmetry.
Is-a
1 item(s)
MI:0495
experimental role
Comments
2 comment(s)
Comment 1
The CTE sequence is found in the 3' UTR of the viral RNA corresponding to 7620-7859 fragment of the M11841 EMBL entry.
Comment 2
Cis-acting sequence interacting with cellular nuclear export proteins to regulate the viral RNA splicing and export.
Experiments (1)
Experiment 1
tang-1997a-3
Full name
A cellular cofactor for the constitutive transport element of type D retrovirus.
Detection method
fluorescence microscopy (MI:0416)
Term ID
Name
fluorescence microscopy
Definition
Fluorescent microscopy uses a high intensity light to illuminate the sample. This light excites fluorescence species in the sample, which then emit light of a longer wavelength. A fluorescent microscope also produces a magnified image of the sample, but the image is based on the second light source -- the light emanating from the fluorescent species -- rather than from the light originally used to illuminate, and excite, the sample.
Synonyms
1 synonym(s)
fluorescence imaging (PSI-MI-short)
Is-a
1 item(s)
MI:0428
imaging technique
Host organisms
1 host organism(s)
Origin
Homo sapiens
NCBI Taxonomy ID
Name
Homo sapiens
Synonyms
2 synonym(s).
Reference
TANG, H, et al. , Science 1997 May;276(5317):1412-5
PMID
Title
A cellular cofactor for the constitutive transport element of type D retrovirus.
Authors
TANG, H, et al.
TANG, H
GAIETTA, G M
FISCHER, W H
ELLISMAN, M H
WONG-STAAL, F
Citation
Science 1997 May;276(5317):1412-5
Attributes
6 attribute(s)
author-list
Tang H., Gaietta GM., Fischer WH., Ellisman MH., Wong-Staal F.
contact-email
fwonfstaal@ucsd.edu
exp-modification
Detection of CTE containing RNA by in-situ hybridization with digoxigenin-labeled antisens RNA probe and immunodetection of helicase A.
figure legend
fig 3
publication year
1997
journal
Science (0036-8075)
References (1)
Reference 1
TANG, H, et al. , Science 1997 May;276(5317):1412-5
PMID
Title
A cellular cofactor for the constitutive transport element of type D retrovirus.
Authors
TANG, H, et al.
TANG, H
GAIETTA, G M
FISCHER, W H
ELLISMAN, M H
WONG-STAAL, F
Citation
Science 1997 May;276(5317):1412-5
Attributes (3)
Attributes
3 attribute(s)
comment
Imported from IntAct, European Bioinformatics Institute, release date 2010-09-03.
comment
Export of unspliced viral RNA.
kd
0.0
EBI-867344
MI:0403
1660
GO:0000166
8690889
9606
Q08211
NP_001348.2
REACT_6167
IPR014001
ENSG00000135829
IPI00844578
dhx9_human
M11841