Interaction information

Interaction summary

Short label

Glutaminyl-tRNA synthetase-Glutaminyl-tRNA

Full name

Glutaminyl-tRNA synthetase interacts with Glutaminyl-tRNA

Source database

RCSB PDB

ID

1ZJW

Date modified

2011-03-24 18:45:44 JST

Protein information

Short label

Glutaminyl-tRNA synthetase

Full name

Chain A of model 1ZJW: Glutaminyl-tRNA synthetase

Gene

glnS (glutamyl-tRNA synthetase)

GeneID

945310

Official symbol

glnS

Synonyms

2 synonym(s)

• ECK0668
• JW0666

Description

glutamyl-tRNA synthetase

Ontologies

2 ontology(s)

Functions

0 function(s)

Processes

1 process(s)

GO:0006418

tRNA aminoacylation for protein translation

Evidence

n/a

Term ID

GO:0006418

Name

tRNA aminoacylation for protein translation

Namespace

biological_process

Definition

The synthesis of aminoacyl tRNA by the formation of an ester bond between the 3'-hydroxyl group of the most 3' adenosine of the tRNA, to be used in ribosome-mediated polypeptide synthesis.

Synonyms

1 synonym(s)

• tRNA charging

Is-a

1 item(s)

GO:0043039

tRNA aminoacylation

Components

1 component(s)

GO:0005737

cytoplasm

Evidence

n/a

Term ID

GO:0005737

Name

cytoplasm

Namespace

cellular_component

Definition

All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.

Is-a

1 item(s)

GO:0044424

intracellular part

Origin

Escherichia coli

NCBI Taxonomy ID

562

Name

Escherichia coli

Synonyms

16 synonym(s).

• "Bacillus coli" Migula 1895
• "Bacterium coli commune" Escherich 1885
• "Bacterium coli" (Migula 1895) Lehmann and Neumann 1896
• Bacillus coli
• Bacterium coli
• Bacterium coli commune
• Enterococcus coli
• Escherchia coli
• Escherichia coli (Migula 1895) Castellani and Chalmers 1919
• Escherichia coli retron Ec107
• Escherichia coli retron Ec67
• Escherichia coli retron Ec79
• Escherichia coli retron Ec86
• Eschericia coli
• bacterium 10a
• bacterium E3

Cross references

1 item(s)

UniProt

P00962

Sequence

1ZJWA:Glutaminyl-tRNA synthetase

RNA information

Short label

Glutaminyl-tRNA

Full name

Chain B of model 1ZJW: Glutaminyl-tRNA

Sequence

1ZJWB:Glutaminyl-tRNA

Experiments (1)

Experiment 1

gruic-2005

Full name

X-ray diffraction

Detection method

x-ray crystallography (MI:0114)

Term ID

MI:0114

Name

x-ray crystallography

Definition

Analysis of a diffraction pattern generated by a single crystal. X-rays have a wavelength, typically around 1 Angstrom (the diameter of a hydrogen atom). If a narrow parallel beam of X-rays is directed at a sample of a pure protein, most of the X-rays will pass straight through it. A small fraction, however, will be scattered by the atoms in the sample. If the sample is a well-ordered crystal, the scattered waves will reinforce one another at certain points and will appear as diffraction spots when the X-rays are recorded by a suitable detector. The position and intensity of each spot in the X-ray diffraction pattern contain information about the position and nature of the atoms in the crystal. The three-dimensional structure of a large molecule can be deduced from the electron-density map of its crystal. In recent years X-ray diffraction analysis has become increasingly automated, and now the slowest step is likely to be the production of suitable macromolecule crystals. This requires high concentration of very pure macromolecule and empirical searching for the proper crystallisation conditions.

Synonyms

2 synonym(s)

• X-ray (PSI-MI-alternate)
• x-ray diffraction (PSI-MI-short)

Is-a

2 item(s)

MI:0013

biophysical

MI:0659

experimental feature detection

Host organisms

1 host organism(s)

Origin

Escherichia coli

NCBI Taxonomy ID

562

Name

Escherichia coli

Synonyms

16 synonym(s).

• "Bacillus coli" Migula 1895
• "Bacterium coli commune" Escherich 1885
• "Bacterium coli" (Migula 1895) Lehmann and Neumann 1896
• Bacillus coli
• Bacterium coli
• Bacterium coli commune
• Enterococcus coli
• Escherchia coli
• Escherichia coli (Migula 1895) Castellani and Chalmers 1919
• Escherichia coli retron Ec107
• Escherichia coli retron Ec67
• Escherichia coli retron Ec79
• Escherichia coli retron Ec86
• Eschericia coli
• bacterium 10a
• bacterium E3

Reference

GRUIC-SOVULJ, Ita, et al., J. Biol. Chem. 2005 Jun;280(25):23978-86

PMID

15845536

Title

tRNA-dependent aminoacyl-adenylate hydrolysis by a nonediting class I aminoacyl-tRNA synthetase.

Authors

GRUIC-SOVULJ, Ita, et al.

• GRUIC-SOVULJ, Ita
• UTER, Nathan
• BULLOCK, Timothy
• PERONA, John J

Citation

J. Biol. Chem. 2005 Jun;280(25):23978-86

References (1)

Reference 1

GRUIC-SOVULJ, Ita, et al., J. Biol. Chem. 2005 Jun;280(25):23978-86

PMID

15845536

Title

tRNA-dependent aminoacyl-adenylate hydrolysis by a nonediting class I aminoacyl-tRNA synthetase.

Authors

GRUIC-SOVULJ, Ita, et al.

• GRUIC-SOVULJ, Ita
• UTER, Nathan
• BULLOCK, Timothy
• PERONA, John J

Citation

J. Biol. Chem. 2005 Jun;280(25):23978-86

Related articles (1)

Article 1

GRUIC-SOVULJ, Ita, et al., J. Biol. Chem. 2005 Jun;280(25):23978-86

PMID

15845536

Title

tRNA-dependent aminoacyl-adenylate hydrolysis by a nonediting class I aminoacyl-tRNA synthetase.

Authors

GRUIC-SOVULJ, Ita, et al.

• GRUIC-SOVULJ, Ita
• UTER, Nathan
• BULLOCK, Timothy
• PERONA, John J

Citation

J. Biol. Chem. 2005 Jun;280(25):23978-86

Attributes (3)

Attributes

3 attribute(s)

3d-structure

Glutaminyl-tRNA synthetase complexed to glutamine and 2'deoxy A76 glutamine tRNA

3d-resolution

2.500A

3d-r-factors

working 22.000%, free 24.900%