Your browser does not support JavaScript or JavaScript is disabled. This site does not function correctly with JavaScript disabled.
Interaction information
Interaction summary
Short label
Ribosomal large subunit pseudouridine synthase A-5'-R(*GP*AP*GP*GP*GP*GP*AP*UP*UP*GP*AP*AP*AP*AP*UP*CP*CP*CP*CP*UP*C)-3'
Full name
Ribosomal large subunit pseudouridine synthase A interacts with 5'-R(*GP*AP*GP*GP*GP*GP*AP*UP*UP*GP*AP*AP*AP*AP*UP*CP*CP*CP*CP*UP*C)-3'
Source database
RCSB PDB ID
Date modified
2011-03-24 21:37:07 JST
Protein information
Short label
Ribosomal large subunit pseudouridine synthase A
Full name
Chain A,B,C,D of model 2I82: Ribosomal large subunit pseudouridine synthase A
Gene
rluA (pseudouridine synthase for 23S rRNA (position 746) and tRNAphe(position 32))
GeneID
Official symbol
rluA
Synonyms
3 synonym(s)
Description
pseudouridine synthase for 23S rRNA (position 746) and tRNAphe(position 32)
Ontologies
1 ontology(s)
Functions
0 function(s)
Processes
1 process(s)
GO:0009451
RNA modification
Evidence
n/a
Term ID
Name
RNA modification
Namespace
biological_process
Definition
The covalent alteration of one or more nucleotides within an RNA molecule to produce an RNA molecule with a sequence that differs from that coded genetically." [GOC:go_curators, ISBN:1555811337 "Modification and Editing of RNA
Synonyms
1 synonym(s)
Is-a
2 item(s)
GO:0016070
RNA metabolic process
GO:0043412
macromolecule modification
Components
0 component(s)
Origin
Escherichia coli
NCBI Taxonomy ID
Name
Escherichia coli
Synonyms
16 synonym(s).
"Bacillus coli" Migula 1895
"Bacterium coli commune" Escherich 1885
"Bacterium coli" (Migula 1895) Lehmann and Neumann 1896
Bacillus coli
Bacterium coli
Bacterium coli commune
Enterococcus coli
Escherchia coli
Escherichia coli (Migula 1895) Castellani and Chalmers 1919
Escherichia coli retron Ec107
Escherichia coli retron Ec67
Escherichia coli retron Ec79
Escherichia coli retron Ec86
Eschericia coli
bacterium 10a
bacterium E3
Cross references
1 item(s)
UniProt
Sequence
RNA information
Short label
5'-R(*GP*AP*GP*GP*GP*GP*AP*UP*UP*GP*AP*AP*AP*AP*UP*CP*CP*CP*CP*UP*C)-3'
Full name
Chain E,F,G,H of model 2I82: 5'-R(*GP*AP*GP*GP*GP*GP*AP*UP*UP*GP*AP*AP*AP*AP*UP*CP*CP*CP*CP*UP*C)-3'
Sequence
Experiments (1)
Experiment 1
hoang-2006
Full name
X-ray diffraction
Detection method
x-ray crystallography (MI:0114)
Term ID
Name
x-ray crystallography
Definition
Analysis of a diffraction pattern generated by a single crystal. X-rays have a wavelength, typically around 1 Angstrom (the diameter of a hydrogen atom). If a narrow parallel beam of X-rays is directed at a sample of a pure protein, most of the X-rays will pass straight through it. A small fraction, however, will be scattered by the atoms in the sample. If the sample is a well-ordered crystal, the scattered waves will reinforce one another at certain points and will appear as diffraction spots when the X-rays are recorded by a suitable detector. The position and intensity of each spot in the X-ray diffraction pattern contain information about the position and nature of the atoms in the crystal. The three-dimensional structure of a large molecule can be deduced from the electron-density map of its crystal. In recent years X-ray diffraction analysis has become increasingly automated, and now the slowest step is likely to be the production of suitable macromolecule crystals. This requires high concentration of very pure macromolecule and empirical searching for the proper crystallisation conditions.
Synonyms
2 synonym(s)
X-ray (PSI-MI-alternate)
x-ray diffraction (PSI-MI-short)
Is-a
2 item(s)
MI:0013
biophysical
MI:0659
experimental feature detection
Host organisms
1 host organism(s)
Origin
Escherichia coli BL21(DE3)
NCBI Taxonomy ID
Name
Escherichia coli BL21(DE3)
Synonyms
2 synonym(s).
Escherichia coli str. BL21(DE3)
Escherichia coli strain BL21(DE3)
Reference
HOANG, Charmaine, et al. , Mol. Cell 2006 Nov;24(4):535-45
PMID
Title
Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure.
Authors
HOANG, Charmaine, et al.
HOANG, Charmaine
CHEN, Junjun
VIZTHUM, Caroline A
KANDEL, Jason M
HAMILTON, Christopher S
MUELLER, Eugene G
FERRé-D'AMARé, Adrian R
Citation
Mol. Cell 2006 Nov;24(4):535-45
References (1)
Reference 1
HOANG, Charmaine, et al. , Mol. Cell 2006 Nov;24(4):535-45
PMID
Title
Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure.
Authors
HOANG, Charmaine, et al.
HOANG, Charmaine
CHEN, Junjun
VIZTHUM, Caroline A
KANDEL, Jason M
HAMILTON, Christopher S
MUELLER, Eugene G
FERRé-D'AMARé, Adrian R
Citation
Mol. Cell 2006 Nov;24(4):535-45
Related articles (1)
Article 1
HOANG, Charmaine, et al. , Mol. Cell 2006 Nov;24(4):535-45
PMID
Title
Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure.
Authors
HOANG, Charmaine, et al.
HOANG, Charmaine
CHEN, Junjun
VIZTHUM, Caroline A
KANDEL, Jason M
HAMILTON, Christopher S
MUELLER, Eugene G
FERRé-D'AMARé, Adrian R
Citation
Mol. Cell 2006 Nov;24(4):535-45
Attributes (3)
Attributes
3 attribute(s)
3d-structure
Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure
3d-resolution
2.05A
3d-r-factors
working 23.400%, free 26.300%
2I82
946262
GO:0009451
562
P0AA37
2I82A:Ribosomal large subunit pseudouridine synthase A
MI:0114
17188032