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Interaction information
Interaction summary
Short label
PRP24-LSM4
Source database
BioGRID
ID
206349
Type
physical association (MI:0915)
Term ID
MI:0915
Name
physical association
Definition
Molecules that are experimentally shown to belong to the same functional or structural complex.
Is-a
1 item(s)
MI:0914
association
Date modified
2011-03-19 23:40:58 JST
Protein information
Short label
PRP24
Gene
PRP24 (Prp24p)
GeneID
855310
Official symbol
PRP24
Description
Prp24p
Other names
1 designation(s)
Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling
Ontologies
11 ontology(s)
Functions
5 function(s)
GO:0000166
[IEA] nucleotide binding
Evidence
IEA
Term ID
GO:0000166
Name
nucleotide binding
Namespace
molecular_function
Definition
Interacting selectively and non-covalently with a nucleotide, any compound consisting of a nucleoside that is esterified with (ortho)phosphate or an oligophosphate at any hydroxyl group on the ribose or deoxyribose moiety.
Is-a
1 item(s)
GO:0005488
binding
GO:0003676
[IEA] nucleic acid binding
Evidence
IEA
Term ID
GO:0003676
Name
nucleic acid binding
Namespace
molecular_function
Definition
Interacting selectively and non-covalently with any nucleic acid.
Is-a
1 item(s)
GO:0005488
binding
GO:0003723
[IEA] RNA binding
Evidence
IEA
Term ID
GO:0003723
Name
RNA binding
Namespace
molecular_function
Definition
Interacting selectively and non-covalently with an RNA molecule or a portion thereof.
Is-a
1 item(s)
GO:0003676
nucleic acid binding
GO:0017069
[IPI] snRNA binding
Evidence
IPI
References
1 reference(s)
PubMed
KARADUMAN, Ramazan, et al. , J. Mol. Biol. 2006 Mar;356(5):1248-62
PMID
16410014
Title
RNA structure and RNA-protein interactions in purified yeast U6 snRNPs.
Authors
KARADUMAN, Ramazan, et al.
KARADUMAN, Ramazan
FABRIZIO, Patrizia
HARTMUTH, Klaus
URLAUB, Henning
LüHRMANN, Reinhard
Citation
J. Mol. Biol. 2006 Mar;356(5):1248-62
Term ID
GO:0017069
Name
snRNA binding
Namespace
molecular_function
Definition
Interacting selectively and non-covalently with a small nuclear RNA (snRNA).
Synonyms
1 synonym(s)
small nuclear RNA binding
Is-a
1 item(s)
GO:0003723
RNA binding
GO:0017070
[IDA] U6 snRNA binding
Evidence
IDA
Term ID
GO:0017070
Name
U6 snRNA binding
Namespace
molecular_function
Definition
Interacting selectively and non-covalently with the U6 small nuclear RNA (U6 snRNA).
Is-a
1 item(s)
GO:0017069
snRNA binding
Processes
4 process(s)
GO:0000244
[IDA] assembly of spliceosomal tri-snRNP
Evidence
IDA
References
1 reference(s)
PubMed
RAGHUNATHAN, P L, et al. , Science 1998 Feb;279(5352):857-60
PMID
9452384
Title
A spliceosomal recycling factor that reanneals U4 and U6 small nuclear ribonucleoprotein particles.
Authors
RAGHUNATHAN, P L, et al.
RAGHUNATHAN, P L
GUTHRIE, C
Citation
Science 1998 Feb;279(5352):857-60
Term ID
GO:0000244
Name
assembly of spliceosomal tri-snRNP
Namespace
biological_process
Definition
The formation of a tri-snRNP complex containing U4 and U6 (or U4atac and U6atac) snRNAs and U5 snRNAs and associated proteins. This includes reannealing of U4 and U6 (or U4atac and U6atac) snRNAs released from previous rounds of splicing to reform the U4/U6 snRNP (or U4atac/U6atac snRNP) as well as the subsequent association of the U5 snRNP with the U4/U6 snRNP (or U4atac/U6atac snRNP) to form a tri-snRNP that is ready to reassemble into another spliceosome complex.
Synonyms
6 synonym(s)
assembly of spliceosomal tri-snRNP U4/U6.U5
assembly of spliceosomal tri-snRNP U4atac/U6atac.U5
snRNP recycling
spliceosomal tri-snRNP assembly
spliceosomal tri-snRNP U4/U6.U5 assembly
spliceosomal tri-snRNP U4atac/U6atac.U5 assembly
Is-a
1 item(s)
GO:0022618
ribonucleoprotein complex assembly
GO:0000245
[IMP] spliceosome assembly
Evidence
IMP
References
1 reference(s)
PubMed
LYGEROU, Z, et al. , Mol. Cell. Biol. 1999 Mar;19(3):2008-20
PMID
10022888
Title
A novel genetic screen for snRNP assembly factors in yeast identifies a conserved protein, Sad1p, also required for pre-mRNA splicing.
Authors
LYGEROU, Z, et al.
LYGEROU, Z
CHRISTOPHIDES, G
SéRAPHIN, B
Citation
Mol. Cell. Biol. 1999 Mar;19(3):2008-20
Term ID
GO:0000245
Name
spliceosome assembly
Namespace
biological_process
Definition
The aggregation, arrangement and bonding together of the spliceosome, a ribonucleoprotein apparatus that catalyzes nuclear mRNA splicing via transesterification reactions.
Is-a
1 item(s)
GO:0022618
ribonucleoprotein complex assembly
GO:0006397
[IEA] mRNA processing
Evidence
IEA
Term ID
GO:0006397
Name
mRNA processing
Namespace
biological_process
Definition
Any process involved in the conversion of a primary mRNA transcript into one or more mature mRNA(s) prior to translation into polypeptide.
Synonyms
1 synonym(s)
Is-a
2 item(s)
GO:0006396
RNA processing
GO:0016071
mRNA metabolic process
GO:0008380
[IEA] RNA splicing
Evidence
IEA
Term ID
GO:0008380
Name
RNA splicing
Namespace
biological_process
Definition
The process of removing sections of the primary RNA transcript to remove sequences not present in the mature form of the RNA and joining the remaining sections to form the mature form of the RNA.
Synonyms
1 synonym(s)
pre-mRNA splicing factor activity
Is-a
1 item(s)
GO:0006396
RNA processing
Components
2 component(s)
GO:0005634
[IEA] nucleus
Evidence
IEA
Term ID
GO:0005634
Name
nucleus
Namespace
cellular_component
Definition
A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
Synonyms
1 synonym(s)
Is-a
1 item(s)
GO:0043231
intracellular membrane-bounded organelle
GO:0005688
[IDA] U6 snRNP
Evidence
IDA
References
1 reference(s)
PubMed
STEVENS, S W, et al. , RNA 2001 Nov;7(11):1543-53
PMID
11720284
Title
Biochemical and genetic analyses of the U5, U6, and U4/U6 x U5 small nuclear ribonucleoproteins from Saccharomyces cerevisiae.
Authors
STEVENS, S W, et al.
STEVENS, S W
BARTA, I
GE, H Y
MOORE, R E
YOUNG, M K
LEE, T D
ABELSON, J
Citation
RNA 2001 Nov;7(11):1543-53
Term ID
GO:0005688
Name
U6 snRNP
Namespace
cellular_component
Definition
A ribonucleoprotein complex that contains small nuclear RNA U6.
Synonyms
1 synonym(s)
Is-a
1 item(s)
GO:0030532
small nuclear ribonucleoprotein complex
Origin
Saccharomyces cerevisiae S288c
NCBI Taxonomy ID
559292
Name
Saccharomyces cerevisiae S288c
Cross references
9 item(s)
RefSeq
NP_013995
Entrez Gene
855310
SGD
S000004881
UniProt
P49960
BioGRID
35446
GenBank Nucleotide
296147388
GenBank Protein
DAA10168
GenBank Protein
6323924
GenBank Protein
285814273
Experimental roles
1 role(s)
Role 1
bait
Term ID
MI:0496
Name
bait
Definition
Molecule experimentally treated to capture its interacting partners.
Is-a
1 item(s)
MI:0495
experimental role
Identification methods
1 identification method(s)
Method 1
predetermined participant
Term ID
MI:0396
Name
predetermined participant
Definition
Molecule whose sequence identity is not checked and has been assumed from external or previous experimental evidence(s).
Synonyms
1 synonym(s)
predetermined (PSI-MI-short)
Is-a
1 item(s)
MI:0661
experimental participant identification
RNA information
Short label
LSM4
Gene
LSM4 (Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA)
GeneID
856848
Official symbol
LSM4
Synonyms
2 synonym(s)
Description
Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
Other names
1 designation(s)
Ontologies
22 ontology(s)
Functions
3 function(s)
GO:0003723
[IEA] RNA binding
Evidence
IEA
Term ID
GO:0003723
Name
RNA binding
Namespace
molecular_function
Definition
Interacting selectively and non-covalently with an RNA molecule or a portion thereof.
Is-a
1 item(s)
GO:0003676
nucleic acid binding
GO:0017070
[IDA] U6 snRNA binding
Evidence
IDA
References
1 reference(s)
PubMed
SALGADO-GARRIDO, J, et al. , EMBO J. 1999 Jun;18(12):3451-62
PMID
10369684
Title
Sm and Sm-like proteins assemble in two related complexes of deep evolutionary origin.
Authors
SALGADO-GARRIDO, J, et al.
SALGADO-GARRIDO, J
BRAGADO-NILSSON, E
KANDELS-LEWIS, S
SéRAPHIN, B
Citation
EMBO J. 1999 Jun;18(12):3451-62
Term ID
GO:0017070
Name
U6 snRNA binding
Namespace
molecular_function
Definition
Interacting selectively and non-covalently with the U6 small nuclear RNA (U6 snRNA).
Is-a
1 item(s)
GO:0017069
snRNA binding
GO:0017070
[IMP] U6 snRNA binding
Evidence
IMP
References
1 reference(s)
PubMed
SALGADO-GARRIDO, J, et al. , EMBO J. 1999 Jun;18(12):3451-62
PMID
10369684
Title
Sm and Sm-like proteins assemble in two related complexes of deep evolutionary origin.
Authors
SALGADO-GARRIDO, J, et al.
SALGADO-GARRIDO, J
BRAGADO-NILSSON, E
KANDELS-LEWIS, S
SéRAPHIN, B
Citation
EMBO J. 1999 Jun;18(12):3451-62
Term ID
GO:0017070
Name
U6 snRNA binding
Namespace
molecular_function
Definition
Interacting selectively and non-covalently with the U6 small nuclear RNA (U6 snRNA).
Is-a
1 item(s)
GO:0017069
snRNA binding
Processes
9 process(s)
GO:0000398
[IPI] nuclear mRNA splicing, via spliceosome
Evidence
IPI
References
1 reference(s)
PubMed
STEVENS, S W, et al. , Proc. Natl. Acad. Sci. U.S.A. 1999 Jun;96(13):7226-31
PMID
10377396
Title
Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins.
Authors
STEVENS, S W, et al.
Citation
Proc. Natl. Acad. Sci. U.S.A. 1999 Jun;96(13):7226-31
Term ID
GO:0000398
Name
nuclear mRNA splicing, via spliceosome
Namespace
biological_process
Definition
The joining together of exons from one or more primary transcripts of nuclear messenger RNA (mRNA) and the excision of intron sequences, via a spliceosomal mechanism, so that mRNA consisting only of the joined exons is produced." [GOC:krc, ISBN:0198506732 "Oxford Dictionary of Biochemistry and Molecular Biology
Synonyms
6 synonym(s)
mRNA splicing
nuclear mRNA splicing via U12-type spliceosome
nuclear mRNA splicing via U2-type spliceosome
pre-mRNA splicing
splicing AT-AC intron
splicing GT-AG intron
Is-a
2 item(s)
GO:0000377
RNA splicing, via transesterification reactions with bulged adenosine as nucleophile
GO:0006397
mRNA processing
GO:0000398
[TAS] nuclear mRNA splicing, via spliceosome
Evidence
TAS
References
1 reference(s)
PubMed
FERNANDEZ, Cesar F, et al. , Mol. Biol. Cell 2004 Jun;15(6):2842-52
PMID
15075370
Title
An Lsm2-Lsm7 complex in Saccharomyces cerevisiae associates with the small nucleolar RNA snR5.
Authors
FERNANDEZ, Cesar F, et al.
FERNANDEZ, Cesar F
PANNONE, Barbara K
CHEN, Xinguo
FUCHS, Gabriele
WOLIN, Sandra L
Citation
Mol. Biol. Cell 2004 Jun;15(6):2842-52
Term ID
GO:0000398
Name
nuclear mRNA splicing, via spliceosome
Namespace
biological_process
Definition
The joining together of exons from one or more primary transcripts of nuclear messenger RNA (mRNA) and the excision of intron sequences, via a spliceosomal mechanism, so that mRNA consisting only of the joined exons is produced." [GOC:krc, ISBN:0198506732 "Oxford Dictionary of Biochemistry and Molecular Biology
Synonyms
6 synonym(s)
mRNA splicing
nuclear mRNA splicing via U12-type spliceosome
nuclear mRNA splicing via U2-type spliceosome
pre-mRNA splicing
splicing AT-AC intron
splicing GT-AG intron
Is-a
2 item(s)
GO:0000377
RNA splicing, via transesterification reactions with bulged adenosine as nucleophile
GO:0006397
mRNA processing
GO:0000956
[TAS] nuclear-transcribed mRNA catabolic process
Evidence
TAS
References
1 reference(s)
PubMed
FERNANDEZ, Cesar F, et al. , Mol. Biol. Cell 2004 Jun;15(6):2842-52
PMID
15075370
Title
An Lsm2-Lsm7 complex in Saccharomyces cerevisiae associates with the small nucleolar RNA snR5.
Authors
FERNANDEZ, Cesar F, et al.
FERNANDEZ, Cesar F
PANNONE, Barbara K
CHEN, Xinguo
FUCHS, Gabriele
WOLIN, Sandra L
Citation
Mol. Biol. Cell 2004 Jun;15(6):2842-52
Term ID
GO:0000956
Name
nuclear-transcribed mRNA catabolic process
Namespace
biological_process
Definition
The chemical reactions and pathways resulting in the breakdown of nuclear-transcribed mRNAs in eukaryotic cells.
Synonyms
3 synonym(s)
nuclear mRNA breakdown
nuclear mRNA catabolism
nuclear mRNA degradation
Is-a
1 item(s)
GO:0006402
mRNA catabolic process
GO:0006364
[IEA] rRNA processing
Evidence
IEA
Term ID
GO:0006364
Name
rRNA processing
Namespace
biological_process
Definition
Any process involved in the conversion of a primary ribosomal RNA (rRNA) transcript into one or more mature rRNA molecules.
Synonyms
1 synonym(s)
35S primary transcript processing
Is-a
2 item(s)
GO:0016072
rRNA metabolic process
GO:0034470
ncRNA processing
GO:0006397
[IEA] mRNA processing
Evidence
IEA
Term ID
GO:0006397
Name
mRNA processing
Namespace
biological_process
Definition
Any process involved in the conversion of a primary mRNA transcript into one or more mature mRNA(s) prior to translation into polypeptide.
Synonyms
1 synonym(s)
Is-a
2 item(s)
GO:0006396
RNA processing
GO:0016071
mRNA metabolic process
GO:0008033
[IEA] tRNA processing
Evidence
IEA
Term ID
GO:0008033
Name
tRNA processing
Namespace
biological_process
Definition
The process by which a pre-tRNA molecule is converted to a mature tRNA, ready for addition of an aminoacyl group.
Synonyms
1 synonym(s)
Is-a
2 item(s)
GO:0006399
tRNA metabolic process
GO:0034470
ncRNA processing
GO:0008380
[IEA] RNA splicing
Evidence
IEA
Term ID
GO:0008380
Name
RNA splicing
Namespace
biological_process
Definition
The process of removing sections of the primary RNA transcript to remove sequences not present in the mature form of the RNA and joining the remaining sections to form the mature form of the RNA.
Synonyms
1 synonym(s)
pre-mRNA splicing factor activity
Is-a
1 item(s)
GO:0006396
RNA processing
GO:0033962
[IGI] cytoplasmic mRNA processing body assembly
Evidence
IGI
References
1 reference(s)
PubMed
DECKER, Carolyn J, et al. , J. Cell Biol. 2007 Nov;179(3):437-49
PMID
17984320
Title
Edc3p and a glutamine/asparagine-rich domain of Lsm4p function in processing body assembly in Saccharomyces cerevisiae.
Authors
DECKER, Carolyn J, et al.
DECKER, Carolyn J
TEIXEIRA, Daniela
PARKER, Roy
Citation
J. Cell Biol. 2007 Nov;179(3):437-49
Term ID
GO:0033962
Name
cytoplasmic mRNA processing body assembly
Namespace
biological_process
Definition
The aggregation, arrangement and bonding together of proteins and RNA molecules to form a cytoplasmic mRNA processing body.
Synonyms
3 synonym(s)
P body assembly
P body biogenesis
P-body assembly
Is-a
1 item(s)
GO:0022618
ribonucleoprotein complex assembly
GO:0033962
[IMP] cytoplasmic mRNA processing body assembly
Evidence
IMP
References
1 reference(s)
PubMed
BUCHAN, J Ross, et al. , J. Cell Biol. 2008 Nov;183(3):441-55
PMID
18981231
Title
P bodies promote stress granule assembly in Saccharomyces cerevisiae.
Authors
BUCHAN, J Ross, et al.
BUCHAN, J Ross
MUHLRAD, Denise
PARKER, Roy
Citation
J. Cell Biol. 2008 Nov;183(3):441-55
Term ID
GO:0033962
Name
cytoplasmic mRNA processing body assembly
Namespace
biological_process
Definition
The aggregation, arrangement and bonding together of proteins and RNA molecules to form a cytoplasmic mRNA processing body.
Synonyms
3 synonym(s)
P body assembly
P body biogenesis
P-body assembly
Is-a
1 item(s)
GO:0022618
ribonucleoprotein complex assembly
Components
10 component(s)
GO:0000932
[IDA] cytoplasmic mRNA processing body
Evidence
IDA
References
1 reference(s)
PubMed
REIJNS, Martin A M, et al. , J. Cell. Sci. 2008 Aug;121(Pt 15):2463-72
PMID
18611963
Title
A role for Q/N-rich aggregation-prone regions in P-body localization.
Authors
REIJNS, Martin A M, et al.
REIJNS, Martin A M
ALEXANDER, Ross D
SPILLER, Michael P
BEGGS, Jean D
Citation
J. Cell. Sci. 2008 Aug;121(Pt 15):2463-72
Term ID
GO:0000932
Name
cytoplasmic mRNA processing body
Namespace
cellular_component
Definition
A focus in the cytoplasm where mRNAs may become inactivated by decapping or some other mechanism. mRNA processing and binding proteins are localized to these foci.
Synonyms
3 synonym(s)
cytoplasmic foci
P body
P-body
Is-a
2 item(s)
GO:0030529
ribonucleoprotein complex
GO:0044444
cytoplasmic part
GO:0005634
[IEA] nucleus
Evidence
IEA
Term ID
GO:0005634
Name
nucleus
Namespace
cellular_component
Definition
A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
Synonyms
1 synonym(s)
Is-a
1 item(s)
GO:0043231
intracellular membrane-bounded organelle
GO:0005682
[RCA] U5 snRNP
Evidence
RCA
References
1 reference(s)
PubMed
TIAN, Weidong, et al. , Genome Biol. 2008;9 Suppl 1:S7
PMID
18613951
Title
Combining guilt-by-association and guilt-by-profiling to predict Saccharomyces cerevisiae gene function.
Authors
TIAN, Weidong, et al.
TIAN, Weidong
ZHANG, Lan V
TAşAN, Murat
GIBBONS, Francis D
KING, Oliver D
PARK, Julie
WUNDERLICH, Zeba
CHERRY, J Michael
ROTH, Frederick P
Citation
Genome Biol. 2008;9 Suppl 1:S7
Term ID
GO:0005682
Name
U5 snRNP
Namespace
cellular_component
Definition
A ribonucleoprotein complex that contains small nuclear RNA U5.
Synonyms
1 synonym(s)
Is-a
1 item(s)
GO:0030532
small nuclear ribonucleoprotein complex
GO:0005688
[IDA] U6 snRNP
Evidence
IDA
References
1 reference(s)
PubMed
STEVENS, S W, et al. , RNA 2001 Nov;7(11):1543-53
PMID
11720284
Title
Biochemical and genetic analyses of the U5, U6, and U4/U6 x U5 small nuclear ribonucleoproteins from Saccharomyces cerevisiae.
Authors
STEVENS, S W, et al.
STEVENS, S W
BARTA, I
GE, H Y
MOORE, R E
YOUNG, M K
LEE, T D
ABELSON, J
Citation
RNA 2001 Nov;7(11):1543-53
Term ID
GO:0005688
Name
U6 snRNP
Namespace
cellular_component
Definition
A ribonucleoprotein complex that contains small nuclear RNA U6.
Synonyms
1 synonym(s)
Is-a
1 item(s)
GO:0030532
small nuclear ribonucleoprotein complex
GO:0005688
[TAS] U6 snRNP
Evidence
TAS
References
1 reference(s)
PubMed
VIDAL, V P, et al. , RNA 1999 Nov;5(11):1470-81
PMID
10580475
Title
Characterization of U6 snRNA-protein interactions.
Authors
VIDAL, V P, et al.
VIDAL, V P
VERDONE, L
MAYES, A E
BEGGS, J D
Citation
RNA 1999 Nov;5(11):1470-81
Term ID
GO:0005688
Name
U6 snRNP
Namespace
cellular_component
Definition
A ribonucleoprotein complex that contains small nuclear RNA U6.
Synonyms
1 synonym(s)
Is-a
1 item(s)
GO:0030532
small nuclear ribonucleoprotein complex
GO:0005730
[IDA] nucleolus
Evidence
IDA
References
1 reference(s)
PubMed
FERNANDEZ, Cesar F, et al. , Mol. Biol. Cell 2004 Jun;15(6):2842-52
PMID
15075370
Title
An Lsm2-Lsm7 complex in Saccharomyces cerevisiae associates with the small nucleolar RNA snR5.
Authors
FERNANDEZ, Cesar F, et al.
FERNANDEZ, Cesar F
PANNONE, Barbara K
CHEN, Xinguo
FUCHS, Gabriele
WOLIN, Sandra L
Citation
Mol. Biol. Cell 2004 Jun;15(6):2842-52
Term ID
GO:0005730
Name
nucleolus
Namespace
cellular_component
Definition
A small, dense body one or more of which are present in the nucleus of eukaryotic cells. It is rich in RNA and protein, is not bounded by a limiting membrane, and is not seen during mitosis. Its prime function is the transcription of the nucleolar DNA into 45S ribosomal-precursor RNA, the processing of this RNA into 5.8S, 18S, and 28S components of ribosomal RNA, and the association of these components with 5S RNA and proteins synthesized outside the nucleolus. This association results in the formation of ribonucleoprotein precursors; these pass into the cytoplasm and mature into the 40S and 60S subunits of the ribosome." [ISBN:0198506732 "Oxford Dictionary of Biochemistry and Molecular Biology
Is-a
2 item(s)
GO:0043232
intracellular non-membrane-bounded organelle
GO:0044428
nuclear part
GO:0005732
[IPI] small nucleolar ribonucleoprotein complex
Evidence
IPI
References
1 reference(s)
PubMed
FERNANDEZ, Cesar F, et al. , Mol. Biol. Cell 2004 Jun;15(6):2842-52
PMID
15075370
Title
An Lsm2-Lsm7 complex in Saccharomyces cerevisiae associates with the small nucleolar RNA snR5.
Authors
FERNANDEZ, Cesar F, et al.
FERNANDEZ, Cesar F
PANNONE, Barbara K
CHEN, Xinguo
FUCHS, Gabriele
WOLIN, Sandra L
Citation
Mol. Biol. Cell 2004 Jun;15(6):2842-52
Term ID
GO:0005732
Name
small nucleolar ribonucleoprotein complex
Namespace
cellular_component
Definition
A ribonucleoprotein complex that contains a small nucleolar RNA (snoRNA) and associated proteins, and is found in eukaryotic cells. Most are involved in a step of processing of rRNA: cleavage, 2'-O-methylation, or pseudouridylation. The majority, though not all, fall into one of two classes, box C/D type or box H/ACA type.
Synonyms
2 synonym(s)
small nucleolar ribonucleoprotein
snoRNP
Is-a
1 item(s)
GO:0030529
ribonucleoprotein complex
GO:0005737
[IEA] cytoplasm
Evidence
IEA
Term ID
GO:0005737
Name
cytoplasm
Namespace
cellular_component
Definition
All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
Is-a
1 item(s)
GO:0044424
intracellular part
GO:0030529
[IEA] ribonucleoprotein complex
Evidence
IEA
Term ID
GO:0030529
Name
ribonucleoprotein complex
Namespace
cellular_component
Definition
A macromolecular complex containing both protein and RNA molecules.
Synonyms
4 synonym(s)
protein-RNA complex
ribonucleoprotein
RNA-protein complex
RNP
Is-a
2 item(s)
GO:0032991
macromolecular complex
GO:0044424
intracellular part
GO:0046540
[IDA] U4/U6 x U5 tri-snRNP complex
Evidence
IDA
References
1 reference(s)
PubMed
GOTTSCHALK, A, et al. , EMBO J. 1999 Aug;18(16):4535-48
PMID
10449419
Title
Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP.
Authors
GOTTSCHALK, A, et al.
GOTTSCHALK, A
NEUBAUER, G
BANROQUES, J
MANN, M
LüHRMANN, R
FABRIZIO, P
Citation
EMBO J. 1999 Aug;18(16):4535-48
Term ID
GO:0046540
Name
U4/U6 x U5 tri-snRNP complex
Namespace
cellular_component
Definition
A ribonucleoprotein complex formed by the association of the U4/U6 and U5 small nuclear ribonucleoproteins.
Synonyms
1 synonym(s)
Is-a
1 item(s)
GO:0030532
small nuclear ribonucleoprotein complex
Origin
Saccharomyces cerevisiae S288c
NCBI Taxonomy ID
559292
Name
Saccharomyces cerevisiae S288c
Cross references
9 item(s)
RefSeq
NP_011037
Entrez Gene
856848
SGD
S000000914
UniProt
P40070
BioGRID
36857
GenBank Nucleotide
296144129
GenBank Protein
DAA07772
GenBank Protein
285811744
GenBank Protein
6320958
Experimental roles
1 role(s)
Role 1
prey
Term ID
MI:0498
Name
prey
Definition
Molecule experimentally identified as being captured by a given bait.
Is-a
1 item(s)
MI:0495
experimental role
Identification methods
1 identification method(s)
Method 1
predetermined participant
Term ID
MI:0396
Name
predetermined participant
Definition
Molecule whose sequence identity is not checked and has been assumed from external or previous experimental evidence(s).
Synonyms
1 synonym(s)
predetermined (PSI-MI-short)
Is-a
1 item(s)
MI:0661
experimental participant identification
Experiments (1)
Experiment 1
Karaduman R (2006)
Full name
RNA structure and RNA-protein interactions in purified yeast U6 snRNPs.
Detection method
affinity chromatography technology (MI:0004)
Term ID
MI:0004
Name
affinity chromatography technology
Definition
This class of approaches is characterised by the use of affinity resins as tools to purify molecule of interest (baits) and their binding partners. The baits can be captured by a variety of high affinity ligands linked to a resin - for example, antibodies specific for the bait itself, antibodies for specific tags engineered to be expressed as part of the bait or other high affinity binders such as glutathione resins for GST fusion proteins, metal resins for histidine-tagged proteins.
Synonyms
2 synonym(s)
affinity chrom (PSI-MI-short)
Affinity purification (PSI-MI-alternate)
Is-a
2 item(s)
MI:0091
chromatography technology
MI:0400
affinity technology
Host organisms
1 host organism(s)
Origin
unidentified
NCBI Taxonomy ID
32644
Name
unidentified
Synonyms
11 synonym(s).
miscellaneous nucleic acid
none
not shown
not specified
other
sonstige nucleic acid
unidentified organism
unknown
unknown organism
unknwon
unspecified
Reference
KARADUMAN, Ramazan, et al. , J. Mol. Biol. 2006 Mar;356(5):1248-62
PMID
16410014
Title
RNA structure and RNA-protein interactions in purified yeast U6 snRNPs.
Authors
KARADUMAN, Ramazan, et al.
KARADUMAN, Ramazan
FABRIZIO, Patrizia
HARTMUTH, Klaus
URLAUB, Henning
LüHRMANN, Reinhard
Citation
J. Mol. Biol. 2006 Mar;356(5):1248-62
References (1)
Reference 1
KARADUMAN, Ramazan, et al. , J. Mol. Biol. 2006 Mar;356(5):1248-62
PMID
16410014
Title
RNA structure and RNA-protein interactions in purified yeast U6 snRNPs.
Authors
KARADUMAN, Ramazan, et al.
KARADUMAN, Ramazan
FABRIZIO, Patrizia
HARTMUTH, Klaus
URLAUB, Henning
LüHRMANN, Reinhard
Citation
J. Mol. Biol. 2006 Mar;356(5):1248-62
Attributes (2)
Attributes
2 attribute(s)
comment
Imported from BioGRID, BIOGRID: All Interactions for Build 3.1.74, release 3.1.74, release date 2011-02-25.
BioGRID Evidence Code
Affinity Capture-RNA