Interaction information

Interaction summary

Short label

SHE2-SRO77

Source database

BioGRID

ID

238112

Type

physical association (MI:0915)

Term ID

MI:0915

Name

physical association

Definition

Molecules that are experimentally shown to belong to the same functional or structural complex.

Is-a

1 item(s)

MI:0914

association

Date modified

2011-03-19 23:44:04 JST

Protein information

Short label

SHE2

Gene

SHE2 (RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud)

GeneID

853728

Official symbol

SHE2

Description

RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud

Other names

1 designation(s)

• She2p

Ontologies

6 ontology(s)

Functions

1 function(s)

GO:0003729

[IDA] mRNA binding

Evidence

IDA

References

1 reference(s)

PubMed

LONG, R M, et al., EMBO J. 2000 Dec;19(23):6592-601

PMID

11101531

Title

She2p is a novel RNA-binding protein that recruits the Myo4p-She3p complex to ASH1 mRNA.

Authors

LONG, R M, et al.

• LONG, R M
• GU, W
• LORIMER, E
• SINGER, R H
• CHARTRAND, P

Citation

EMBO J. 2000 Dec;19(23):6592-601

Term ID

GO:0003729

Name

mRNA binding

Namespace

molecular_function

Definition

Interacting selectively and non-covalently with pre-messenger RNA (pre-mRNA) or messenger RNA (mRNA).

Is-a

1 item(s)

GO:0003723

RNA binding

Processes

3 process(s)

GO:0007029

[RCA] endoplasmic reticulum organization

Evidence

RCA

Term ID

GO:0007029

Name

endoplasmic reticulum organization

Namespace

biological_process

Definition

A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the endoplasmic reticulum.

Synonyms

5 synonym(s)

• endoplasmic reticulum morphology
• endoplasmic reticulum organisation (GOC:curators)
• endoplasmic reticulum organization and biogenesis (GOC:mah)
• ER organisation (GOC:curators)
• ER organization and biogenesis (GOC:mah)

Is-a

1 item(s)

GO:0006996

organelle organization

GO:0007533

[IMP] mating type switching

Evidence

IMP

References

1 reference(s)

PubMed

JANSEN, R P, et al., Cell 1996 Mar;84(5):687-97

PMID

8625407

Title

Mother cell-specific HO expression in budding yeast depends on the unconventional myosin myo4p and other cytoplasmic proteins.

Authors

JANSEN, R P, et al.

• JANSEN, R P
• DOWZER, C
• MICHAELIS, C
• GALOVA, M
• NASMYTH, K

Citation

Cell 1996 Mar;84(5):687-97

Term ID

GO:0007533

Name

mating type switching

Namespace

biological_process

Definition

The conversion of a single-cell organism from one mating type to another by the precise replacement of a DNA sequence at the expressed mating type locus with a copy of a sequence from a donor locus.

Synonyms

1 synonym(s)

• mating type switching and recombination

Is-a

3 item(s)

GO:0006259

DNA metabolic process

GO:0007531

mating type determination

GO:0022413

reproductive process in single-celled organism

GO:0008298

[IDA] intracellular mRNA localization

Evidence

IDA

References

1 reference(s)

PubMed

BöHL, F, et al., EMBO J. 2000 Oct;19(20):5514-24

PMID

11032818

Title

She2p, a novel RNA-binding protein tethers ASH1 mRNA to the Myo4p myosin motor via She3p.

Authors

BöHL, F, et al.

• BöHL, F
• KRUSE, C
• FRANK, A
• FERRING, D
• JANSEN, R P

Citation

EMBO J. 2000 Oct;19(20):5514-24

Term ID

GO:0008298

Name

intracellular mRNA localization

Namespace

biological_process

Definition

Any process by which mRNA is transported to, or maintained in, a specific location within the cell.

Synonyms

4 synonym(s)

• establishment and maintenance of intracellular RNA localization
• intracellular mRNA positioning
• mRNA localization, intracellular
• mRNA positioning, intracellular

Is-a

2 item(s)

GO:0006403

RNA localization

GO:0070727

cellular macromolecule localization

Components

2 component(s)

GO:0005737

[IDA] cytoplasm

Evidence

IDA

References

1 reference(s)

PubMed

JANSEN, R P, et al., Cell 1996 Mar;84(5):687-97

PMID

8625407

Title

Mother cell-specific HO expression in budding yeast depends on the unconventional myosin myo4p and other cytoplasmic proteins.

Authors

JANSEN, R P, et al.

• JANSEN, R P
• DOWZER, C
• MICHAELIS, C
• GALOVA, M
• NASMYTH, K

Citation

Cell 1996 Mar;84(5):687-97

Term ID

GO:0005737

Name

cytoplasm

Namespace

cellular_component

Definition

All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.

Is-a

1 item(s)

GO:0044424

intracellular part

GO:0005934

[IDA] cellular bud tip

Evidence

IDA

References

1 reference(s)

PubMed

BöHL, F, et al., EMBO J. 2000 Oct;19(20):5514-24

PMID

11032818

Title

She2p, a novel RNA-binding protein tethers ASH1 mRNA to the Myo4p myosin motor via She3p.

Authors

BöHL, F, et al.

• BöHL, F
• KRUSE, C
• FRANK, A
• FERRING, D
• JANSEN, R P

Citation

EMBO J. 2000 Oct;19(20):5514-24

Term ID

GO:0005934

Name

cellular bud tip

Namespace

cellular_component

Definition

The end of a cellular bud distal to the site of attachment to the mother cell.

Is-a

1 item(s)

GO:0030427

site of polarized growth

Origin

Saccharomyces cerevisiae S288c

NCBI Taxonomy ID

559292

Name

Saccharomyces cerevisiae S288c

Cross references

9 item(s)

RefSeq

NP_012792

Entrez Gene

853728

SGD

S000001613

UniProt

P36068

BioGRID

34005

GenBank Nucleotide

296146251

GenBank Protein

DAA09031

GenBank Protein

6322719

GenBank Protein

285813134

Experimental roles

1 role(s)

Role 1

bait

Term ID

MI:0496

Name

bait

Definition

Molecule experimentally treated to capture its interacting partners.

Is-a

1 item(s)

MI:0495

experimental role

Identification methods

1 identification method(s)

Method 1

predetermined participant

Term ID

MI:0396

Name

predetermined participant

Definition

Molecule whose sequence identity is not checked and has been assumed from external or previous experimental evidence(s).

Synonyms

1 synonym(s)

• predetermined (PSI-MI-short)

Is-a

1 item(s)

MI:0661

experimental participant identification

RNA information

Short label

SRO77

Gene

SRO77 (Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Sro7p and Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p)

GeneID

852168

Official symbol

SRO77

Synonyms

2 synonym(s)

• SNI2
• SOP2

Description

Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Sro7p and Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p

Other names

1 designation(s)

• Sro77p

Ontologies

7 ontology(s)

Functions

1 function(s)

GO:0003674

[ND] molecular_function

Evidence

ND

Term ID

GO:0003674

Name

molecular_function

Namespace

molecular_function

Definition

Elemental activities, such as catalysis or binding, describing the actions of a gene product at the molecular level. A given gene product may exhibit one or more molecular functions.

Synonyms

2 synonym(s)

• molecular function
• molecular function unknown

Processes

4 process(s)

GO:0006887

[IEA] exocytosis

Evidence

IEA

Term ID

GO:0006887

Name

exocytosis

Namespace

biological_process

Definition

A process of secretion by a cell that results in the release of intracellular molecules (e.g. hormones, matrix proteins) contained within a membrane-bounded vesicle by fusion of the vesicle with the plasma membrane of a cell. This is the process whereby most molecules are secreted from eukaryotic cells.

Synonyms

2 synonym(s)

• nonselective vesicle exocytosis
• vesicle exocytosis

Is-a

2 item(s)

GO:0016192

vesicle-mediated transport

GO:0032940

secretion by cell

GO:0006887

[IGI] exocytosis

Evidence

IGI

References

1 reference(s)

PubMed

LEHMAN, K, et al., J. Cell Biol. 1999 Jul;146(1):125-40

PMID

10402465

Title

Yeast homologues of tomosyn and lethal giant larvae function in exocytosis and are associated with the plasma membrane SNARE, Sec9.

Authors

LEHMAN, K, et al.

• LEHMAN, K
• ROSSI, G
• ADAMO, J E
• BRENNWALD, P

Citation

J. Cell Biol. 1999 Jul;146(1):125-40

Term ID

GO:0006887

Name

exocytosis

Namespace

biological_process

Definition

A process of secretion by a cell that results in the release of intracellular molecules (e.g. hormones, matrix proteins) contained within a membrane-bounded vesicle by fusion of the vesicle with the plasma membrane of a cell. This is the process whereby most molecules are secreted from eukaryotic cells.

Synonyms

2 synonym(s)

• nonselective vesicle exocytosis
• vesicle exocytosis

Is-a

2 item(s)

GO:0016192

vesicle-mediated transport

GO:0032940

secretion by cell

GO:0006893

[IGI] Golgi to plasma membrane transport

Evidence

IGI

References

1 reference(s)

PubMed

LEHMAN, K, et al., J. Cell Biol. 1999 Jul;146(1):125-40

PMID

10402465

Title

Yeast homologues of tomosyn and lethal giant larvae function in exocytosis and are associated with the plasma membrane SNARE, Sec9.

Authors

LEHMAN, K, et al.

• LEHMAN, K
• ROSSI, G
• ADAMO, J E
• BRENNWALD, P

Citation

J. Cell Biol. 1999 Jul;146(1):125-40

Term ID

GO:0006893

Name

Golgi to plasma membrane transport

Namespace

biological_process

Definition

The directed movement of substances from the Golgi to the plasma membrane in transport vesicles that move from the trans-Golgi network to the plasma membrane, where they fuse and release their contents by exocytosis.

Synonyms

1 synonym(s)

• Golgi to plasma membrane vesicle-mediated transport

Is-a

1 item(s)

GO:0006892

post-Golgi vesicle-mediated transport

GO:0006893

[IPI] Golgi to plasma membrane transport

Evidence

IPI

References

1 reference(s)

PubMed

LEHMAN, K, et al., J. Cell Biol. 1999 Jul;146(1):125-40

PMID

10402465

Title

Yeast homologues of tomosyn and lethal giant larvae function in exocytosis and are associated with the plasma membrane SNARE, Sec9.

Authors

LEHMAN, K, et al.

• LEHMAN, K
• ROSSI, G
• ADAMO, J E
• BRENNWALD, P

Citation

J. Cell Biol. 1999 Jul;146(1):125-40

Term ID

GO:0006893

Name

Golgi to plasma membrane transport

Namespace

biological_process

Definition

The directed movement of substances from the Golgi to the plasma membrane in transport vesicles that move from the trans-Golgi network to the plasma membrane, where they fuse and release their contents by exocytosis.

Synonyms

1 synonym(s)

• Golgi to plasma membrane vesicle-mediated transport

Is-a

1 item(s)

GO:0006892

post-Golgi vesicle-mediated transport

Components

2 component(s)

GO:0005886

[IPI] plasma membrane

Evidence

IPI

References

1 reference(s)

PubMed

LEHMAN, K, et al., J. Cell Biol. 1999 Jul;146(1):125-40

PMID

10402465

Title

Yeast homologues of tomosyn and lethal giant larvae function in exocytosis and are associated with the plasma membrane SNARE, Sec9.

Authors

LEHMAN, K, et al.

• LEHMAN, K
• ROSSI, G
• ADAMO, J E
• BRENNWALD, P

Citation

J. Cell Biol. 1999 Jul;146(1):125-40

Term ID

GO:0005886

Name

plasma membrane

Namespace

cellular_component

Definition

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

Synonyms

7 synonym(s)

• bacterial inner membrane
• cell membrane
• cytoplasmic membrane
• inner endospore membrane
• juxtamembrane
• plasma membrane lipid bilayer (GOC:mah)
• plasmalemma

Is-a

1 item(s)

GO:0016020

membrane

GO:0005886

[ISS] plasma membrane

Evidence

ISS

References

1 reference(s)

PubMed

LEHMAN, K, et al., J. Cell Biol. 1999 Jul;146(1):125-40

PMID

10402465

Title

Yeast homologues of tomosyn and lethal giant larvae function in exocytosis and are associated with the plasma membrane SNARE, Sec9.

Authors

LEHMAN, K, et al.

• LEHMAN, K
• ROSSI, G
• ADAMO, J E
• BRENNWALD, P

Citation

J. Cell Biol. 1999 Jul;146(1):125-40

Term ID

GO:0005886

Name

plasma membrane

Namespace

cellular_component

Definition

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

Synonyms

7 synonym(s)

• bacterial inner membrane
• cell membrane
• cytoplasmic membrane
• inner endospore membrane
• juxtamembrane
• plasma membrane lipid bilayer (GOC:mah)
• plasmalemma

Is-a

1 item(s)

GO:0016020

membrane

Origin

Saccharomyces cerevisiae S288c

NCBI Taxonomy ID

559292

Name

Saccharomyces cerevisiae S288c

Cross references

9 item(s)

RefSeq

NP_009444

Entrez Gene

852168

SGD

S000000202

UniProt

P38163

BioGRID

32597

GenBank Nucleotide

296142565

GenBank Protein

DAA07017

GenBank Protein

6319362

GenBank Protein

285810232

Experimental roles

1 role(s)

Role 1

prey

Term ID

MI:0498

Name

prey

Definition

Molecule experimentally identified as being captured by a given bait.

Is-a

1 item(s)

MI:0495

experimental role

Identification methods

1 identification method(s)

Method 1

predetermined participant

Term ID

MI:0396

Name

predetermined participant

Definition

Molecule whose sequence identity is not checked and has been assumed from external or previous experimental evidence(s).

Synonyms

1 synonym(s)

• predetermined (PSI-MI-short)

Is-a

1 item(s)

MI:0661

experimental participant identification

Experiments (1)

Experiment 1

Aronov S (2007)

Full name

mRNAs encoding polarity and exocytosis factors are cotransported with the cortical endoplasmic reticulum to the incipient bud in Saccharomyces cerevisiae.

Detection method

affinity chromatography technology (MI:0004)

Term ID

MI:0004

Name

affinity chromatography technology

Definition

This class of approaches is characterised by the use of affinity resins as tools to purify molecule of interest (baits) and their binding partners. The baits can be captured by a variety of high affinity ligands linked to a resin - for example, antibodies specific for the bait itself, antibodies for specific tags engineered to be expressed as part of the bait or other high affinity binders such as glutathione resins for GST fusion proteins, metal resins for histidine-tagged proteins.

Synonyms

2 synonym(s)

• affinity chrom (PSI-MI-short)
• Affinity purification (PSI-MI-alternate)

Is-a

2 item(s)

MI:0091

chromatography technology

MI:0400

affinity technology

Host organisms

1 host organism(s)

Origin

unidentified

NCBI Taxonomy ID

32644

Name

unidentified

Synonyms

11 synonym(s).

• miscellaneous nucleic acid
• none
• not shown
• not specified
• other
• sonstige nucleic acid
• unidentified organism
• unknown
• unknown organism
• unknwon
• unspecified

Reference

ARONOV, Stella, et al., Mol. Cell. Biol. 2007 May;27(9):3441-55

PMID

17339339

Title

mRNAs encoding polarity and exocytosis factors are cotransported with the cortical endoplasmic reticulum to the incipient bud in Saccharomyces cerevisiae.

Authors

ARONOV, Stella, et al.

• ARONOV, Stella
• GELIN-LICHT, Rita
• ZIPOR, Gadi
• HAIM, Liora
• SAFRAN, Einat
• GERST, Jeffrey E

Citation

Mol. Cell. Biol. 2007 May;27(9):3441-55

References (1)

Reference 1

ARONOV, Stella, et al., Mol. Cell. Biol. 2007 May;27(9):3441-55

PMID

17339339

Title

mRNAs encoding polarity and exocytosis factors are cotransported with the cortical endoplasmic reticulum to the incipient bud in Saccharomyces cerevisiae.

Authors

ARONOV, Stella, et al.

• ARONOV, Stella
• GELIN-LICHT, Rita
• ZIPOR, Gadi
• HAIM, Liora
• SAFRAN, Einat
• GERST, Jeffrey E

Citation

Mol. Cell. Biol. 2007 May;27(9):3441-55

Attributes (2)

Attributes

2 attribute(s)

comment

Imported from BioGRID, BIOGRID: All Interactions for Build 3.1.74, release 3.1.74, release date 2011-02-25.

BioGRID Evidence Code

Affinity Capture-RNA