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Interaction information
Interaction summary
Short label
Signal recognition particle protein-4.5 S RNA
Full name
Signal recognition particle protein interacts with 4.5 S RNA
Source database
RCSB PDB
ID
2PXB
Date modified
2011-03-25 16:22:41 JST
Protein information
Short label
Signal recognition particle protein
Full name
Chain A of model 2PXB: Signal recognition particle protein
Gene
ffh (Signal Recognition Particle (SRP) component with 4.5S RNA (ffs))
GeneID
947102
Official symbol
ffh
Synonyms
2 synonym(s)
Description
Signal Recognition Particle (SRP) component with 4.5S RNA (ffs)
Origin
Escherichia coli
NCBI Taxonomy ID
562
Name
Escherichia coli
Synonyms
16 synonym(s).
"Bacillus coli" Migula 1895
"Bacterium coli commune" Escherich 1885
"Bacterium coli" (Migula 1895) Lehmann and Neumann 1896
Bacillus coli
Bacterium coli
Bacterium coli commune
Enterococcus coli
Escherchia coli
Escherichia coli (Migula 1895) Castellani and Chalmers 1919
Escherichia coli retron Ec107
Escherichia coli retron Ec67
Escherichia coli retron Ec79
Escherichia coli retron Ec86
Eschericia coli
bacterium 10a
bacterium E3
Cross references
1 item(s)
UniProt
P0AGD7
Sequence
2PXBA:Signal recognition particle protein
RNA information
Short label
4.5 S RNA
Full name
Chain B of model 2PXB: 4.5 S RNA
Sequence
2PXBB:4.5 S RNA
Experiments (1)
Experiment 1
keel-2007
Full name
X-ray diffraction
Detection method
x-ray crystallography (MI:0114)
Term ID
MI:0114
Name
x-ray crystallography
Definition
Analysis of a diffraction pattern generated by a single crystal. X-rays have a wavelength, typically around 1 Angstrom (the diameter of a hydrogen atom). If a narrow parallel beam of X-rays is directed at a sample of a pure protein, most of the X-rays will pass straight through it. A small fraction, however, will be scattered by the atoms in the sample. If the sample is a well-ordered crystal, the scattered waves will reinforce one another at certain points and will appear as diffraction spots when the X-rays are recorded by a suitable detector. The position and intensity of each spot in the X-ray diffraction pattern contain information about the position and nature of the atoms in the crystal. The three-dimensional structure of a large molecule can be deduced from the electron-density map of its crystal. In recent years X-ray diffraction analysis has become increasingly automated, and now the slowest step is likely to be the production of suitable macromolecule crystals. This requires high concentration of very pure macromolecule and empirical searching for the proper crystallisation conditions.
Synonyms
2 synonym(s)
X-ray (PSI-MI-alternate)
x-ray diffraction (PSI-MI-short)
Is-a
2 item(s)
MI:0013
biophysical
MI:0659
experimental feature detection
Host organisms
1 host organism(s)
Origin
Escherichia coli
NCBI Taxonomy ID
562
Name
Escherichia coli
Synonyms
16 synonym(s).
"Bacillus coli" Migula 1895
"Bacterium coli commune" Escherich 1885
"Bacterium coli" (Migula 1895) Lehmann and Neumann 1896
Bacillus coli
Bacterium coli
Bacterium coli commune
Enterococcus coli
Escherchia coli
Escherichia coli (Migula 1895) Castellani and Chalmers 1919
Escherichia coli retron Ec107
Escherichia coli retron Ec67
Escherichia coli retron Ec79
Escherichia coli retron Ec86
Eschericia coli
bacterium 10a
bacterium E3
Reference
KEEL, Amanda Y, et al. , Structure 2007 Jul;15(7):761-72
PMID
17637337
Title
A general strategy to solve the phase problem in RNA crystallography.
Authors
KEEL, Amanda Y, et al.
KEEL, Amanda Y
RAMBO, Robert P
BATEY, Robert T
KIEFT, Jeffrey S
Citation
Structure 2007 Jul;15(7):761-72
References (1)
Reference 1
KEEL, Amanda Y, et al. , Structure 2007 Jul;15(7):761-72
PMID
17637337
Title
A general strategy to solve the phase problem in RNA crystallography.
Authors
KEEL, Amanda Y, et al.
KEEL, Amanda Y
RAMBO, Robert P
BATEY, Robert T
KIEFT, Jeffrey S
Citation
Structure 2007 Jul;15(7):761-72
Related articles (1)
Article 1
KEEL, Amanda Y, et al. , Structure 2007 Jul;15(7):761-72
PMID
17637337
Title
A general strategy to solve the phase problem in RNA crystallography.
Authors
KEEL, Amanda Y, et al.
KEEL, Amanda Y
RAMBO, Robert P
BATEY, Robert T
KIEFT, Jeffrey S
Citation
Structure 2007 Jul;15(7):761-72
Attributes (3)
Attributes
3 attribute(s)
3d-structure
Variant 2 of Ribonucleoprotein Core of the E. Coli Signal Recognition Particle
3d-resolution
2.000A
3d-r-factors
working 25.400%, free 27.220%