Interaction information

Interaction summary

Short label

Signal recognition particle protein-4.5 S RNA

Full name

Signal recognition particle protein interacts with 4.5 S RNA

Source database

RCSB PDB

ID

2PXB

Date modified

2011-03-25 16:22:41 JST

Protein information

Short label

Signal recognition particle protein

Full name

Chain A of model 2PXB: Signal recognition particle protein

Gene

ffh (Signal Recognition Particle (SRP) component with 4.5S RNA (ffs))

GeneID

947102

Official symbol

ffh

Synonyms

2 synonym(s)

• ECK2607
• JW5414

Description

Signal Recognition Particle (SRP) component with 4.5S RNA (ffs)

Origin

Escherichia coli

NCBI Taxonomy ID

562

Name

Escherichia coli

Synonyms

16 synonym(s).

• "Bacillus coli" Migula 1895
• "Bacterium coli commune" Escherich 1885
• "Bacterium coli" (Migula 1895) Lehmann and Neumann 1896
• Bacillus coli
• Bacterium coli
• Bacterium coli commune
• Enterococcus coli
• Escherchia coli
• Escherichia coli (Migula 1895) Castellani and Chalmers 1919
• Escherichia coli retron Ec107
• Escherichia coli retron Ec67
• Escherichia coli retron Ec79
• Escherichia coli retron Ec86
• Eschericia coli
• bacterium 10a
• bacterium E3

Cross references

1 item(s)

UniProt

P0AGD7

Sequence

2PXBA:Signal recognition particle protein

RNA information

Short label

4.5 S RNA

Full name

Chain B of model 2PXB: 4.5 S RNA

Sequence

2PXBB:4.5 S RNA

Experiments (1)

Experiment 1

keel-2007

Full name

X-ray diffraction

Detection method

x-ray crystallography (MI:0114)

Term ID

MI:0114

Name

x-ray crystallography

Definition

Analysis of a diffraction pattern generated by a single crystal. X-rays have a wavelength, typically around 1 Angstrom (the diameter of a hydrogen atom). If a narrow parallel beam of X-rays is directed at a sample of a pure protein, most of the X-rays will pass straight through it. A small fraction, however, will be scattered by the atoms in the sample. If the sample is a well-ordered crystal, the scattered waves will reinforce one another at certain points and will appear as diffraction spots when the X-rays are recorded by a suitable detector. The position and intensity of each spot in the X-ray diffraction pattern contain information about the position and nature of the atoms in the crystal. The three-dimensional structure of a large molecule can be deduced from the electron-density map of its crystal. In recent years X-ray diffraction analysis has become increasingly automated, and now the slowest step is likely to be the production of suitable macromolecule crystals. This requires high concentration of very pure macromolecule and empirical searching for the proper crystallisation conditions.

Synonyms

2 synonym(s)

• X-ray (PSI-MI-alternate)
• x-ray diffraction (PSI-MI-short)

Is-a

2 item(s)

MI:0013

biophysical

MI:0659

experimental feature detection

Host organisms

1 host organism(s)

Origin

Escherichia coli

NCBI Taxonomy ID

562

Name

Escherichia coli

Synonyms

16 synonym(s).

• "Bacillus coli" Migula 1895
• "Bacterium coli commune" Escherich 1885
• "Bacterium coli" (Migula 1895) Lehmann and Neumann 1896
• Bacillus coli
• Bacterium coli
• Bacterium coli commune
• Enterococcus coli
• Escherchia coli
• Escherichia coli (Migula 1895) Castellani and Chalmers 1919
• Escherichia coli retron Ec107
• Escherichia coli retron Ec67
• Escherichia coli retron Ec79
• Escherichia coli retron Ec86
• Eschericia coli
• bacterium 10a
• bacterium E3

Reference

KEEL, Amanda Y, et al., Structure 2007 Jul;15(7):761-72

PMID

17637337

Title

A general strategy to solve the phase problem in RNA crystallography.

Authors

KEEL, Amanda Y, et al.

• KEEL, Amanda Y
• RAMBO, Robert P
• BATEY, Robert T
• KIEFT, Jeffrey S

Citation

Structure 2007 Jul;15(7):761-72

References (1)

Reference 1

KEEL, Amanda Y, et al., Structure 2007 Jul;15(7):761-72

PMID

17637337

Title

A general strategy to solve the phase problem in RNA crystallography.

Authors

KEEL, Amanda Y, et al.

• KEEL, Amanda Y
• RAMBO, Robert P
• BATEY, Robert T
• KIEFT, Jeffrey S

Citation

Structure 2007 Jul;15(7):761-72

Related articles (1)

Article 1

KEEL, Amanda Y, et al., Structure 2007 Jul;15(7):761-72

PMID

17637337

Title

A general strategy to solve the phase problem in RNA crystallography.

Authors

KEEL, Amanda Y, et al.

• KEEL, Amanda Y
• RAMBO, Robert P
• BATEY, Robert T
• KIEFT, Jeffrey S

Citation

Structure 2007 Jul;15(7):761-72

Attributes (3)

Attributes

3 attribute(s)

3d-structure

Variant 2 of Ribonucleoprotein Core of the E. Coli Signal Recognition Particle

3d-resolution

2.000A

3d-r-factors

working 25.400%, free 27.220%